The Latest Skin Inflammation Research

The latest inflammation research

The study was conducted by the NICM Health Research Institute & Pharmacology Unit, Western Sydney University

Recently presented by Dr Phoebe Zhou, NICM Western Sydney University, at the ASCC2024 Conference, this new research is now released for all those that could not make the event, and for our global community. This new knowledge is important for anyone formulating with inflammation or Inflammaging in mind.

Discover the four botanicals that performed at therapeutic indexes, demonstrating the importance of working with a botanical's entourage, and then we took it up a level to show our combining entourages (a blend of Native Rivermint, Mountain Pepper Berry and Bitter Orange Cellular Extracts) improved the down regulation of inflammation.

Email us for the Technical Paper to be sent to your inbox and note you would like the Inflammation Research Technical Paper.

SUMMARY

The study, conducted by the NICM Health Research Institute & Pharmacology Unit, Western Sydney University, aimed to investigate the potential of Synergistic NATIVE EXTRACTS Pty Ltd Cellular Extract Concentrates for addressing inflammation and enhancing antioxidant defense, specifically targeting skincare applications.

Abstract:

In skin care, there is a common disparity between the health and the aesthetic, separating the products used as drugs and cosmetics. This disparity, however, can be combined through the usage of several Australian native plants which we have found to demonstrate high levels of natural anti-inflammatory and antioxidant activity in safe dosages within skin care products. Using combinational therapy of NATIVE EXTRACTS (NE) Cellular Extract Concentrate variant supplied by NATIVE EXTRACTS Pty Ltd, three entourages were discovered to produce potent anti-inflammatory and antioxidant activities. These are the NATIVE EXTRACTS Bitter Orange Cellular Extract Concentrate SB P3, the NE Mountain Pepper Berry Cellular Extract Concentrate SB P3, and the NE Native River Mint Cellular Extract Concentrate SB P3. When combined at a ratio of 1:1:1 (w/w), greater potency and synergy were produced in reducing nitric oxide (a major inflammatory marker) than using each extract individually on macrophage cells. The synergy among these NATIVE EXTRACTS Cellular Extract Concentrate variant was substantiated by mathematical models and computer programs generating a combination index value of less than 1. The high antioxidant activity in the three entourages was associated with the upregulation of the antioxidant master regulator, nuclear factor erythroid2-related factor 2 (nrf2), using cellular assays. These results provide preliminary evidence on the natural synergistic and therapeutic value of the NATIVE EXTRACTS NE Cellular Extract Concentrate variants, to address various health conditions including skin health while maintaining its cosmetic value.

Introduction:

Inflammation is implicated in numerous diseases, including skin conditions like eczema and psoriasis. Current treatments often have side effects, prompting the exploration of safer alternatives.

Reactive oxygen species (ROS) contribute to cellular stress, leading to diseases, but the body's antioxidant defense, mediated by Nrf2, can counteract this.

Plant-derived compounds like sulforaphane and curcumin activate Nrf2, making them valuable for health. Combining treatments synergistically enhances their effects, reducing the need for high doses of individual components.

Study objective:

The study aimed to investigate the antioxidant and anti-inflammatory effects of single and combined Synergistic NATIVE EXTRACTS (NE) Cellular Extract Concentrates.

Lab Test Method for Inflammation:

1. NATIVE EXTRACTS Cellular Extracts were prepared and tested for antioxidant and anti-inflammatory effects.

2. Single extracts were tested individually and in combination.

3. Cellular assays were conducted on macrophage and AREc32 cell lines.

4. Various assays like Griess Reagent, ELISA, Luciferase, and Alamar Blue were utilized.

5. The combination index (CI) model was used to determine synergy.

Result:

1. Single NATIVE EXTRACTS Cellular Extracts demonstrated dose-dependent inhibition of nitric oxide (NO), indicating anti-inflammatory activity.

2. The NATIVE EXTRACTS Bitter Orange and NATIVE EXTRACTS Native River Mint Extracts exhibited the strongest NO inhibition, with high therapeutic indexes, indicating safety and efficacy.

3. Compared to other herbal extracts, these NATIVE EXTRACTS Cellular Extracts showed superior therapeutic indexes.

4. Combining NATIVE EXTRACTS Cellular Extracts at a 1:1:1 ratio demonstrated greater anti-inflammatory and antioxidant activity than individual extracts.

5. Mathematical models and computer programs confirmed synergy among these NATIVE EXTRACTS Cellular Extracts.

Dose response curves of nitric oxide production and cell viability on combined NATIVE EXTRACTS Cellular Extract Concentrate variants.

Conclusion: Skin Inflammation Natural Treatment Solution

The study suggests that Synergistic NATIVE EXTRACTS Cellular Extract Concentrates, particularly NATIVE EXTRACTS Native River Mint, Mountain Pepper Berry, and Bitter Orange offer potent anti-inflammatory and antioxidant effects.

Combining these extracts enhances their benefits, making them promising ingredients for skincare products targeting skin health while maintaining cosmetic appeal. This research provides preliminary evidence of their potential therapeutic value in skincare, bridging the gap between health and cosmetic benefits.

Request our ASCC Technical Paper : experience the latest in plant science for the NATIVE EXTRACTS Labs

Sources:

1. Afzal S., Xian Z., Raju R., Or K.W., Muench G. Identification of Nrf2 activators from the roots of Valeriana officinalis. Planta Medica 2022.
2. Asciertom P., Marincola. M. F. Combination therapy: the next opportunity and challenge of medicine. Journal of Translational Medicine 2011, 9, 115, 1-3.
3. Candore G., Bulati M., Caruso C., Castiglia L., Colonna-Romano G., Di Bona D., Duro G., Lio D., Matranga D., Pellicano M. Inflammation, cytokines, immune response, apolipoprotein E, cholesterol, and oxidative stress in Alzheimer disease: therapeutic implications. Rejuvenation Research 2010; 13: 301-313.
4. Jeong W.-S., Jun M., Kong A.-N.T. Nrf2: a potential molecular target for cancer chemoprevention by natural compounds. Antioxidants & redox signaling 2006; 8: 99-106.
5. Pasparakis M., Haase I., Nestle F.O. Mechanisms regulating skin immunity and inflammation. Nature reviews immunology 2014; 14: 289-301.
6. Shyr Z., Cheng Y-S., Lo D., Zheng., W. Drug combination therapy for emerging viral diseases. Drug Discovery Today 2021; 26;10: 2367- 2376
7. Jahnavi K., Reddy P. P., Vasudha B., Narender B. Non-steroidal anti-inflammatory drugs: an overview. Journal of Drug Delivery & Therapeutics 2019; 9;1; 442-448
8. Zhou P., Afzal A., Wohlmuth H., Leach D., Low M., Li G. C. Synergistic Anti-inflammatory Activity of Ginger and Turmeric Extracts in Inhibiting Lipopolysarccharide and Interferon-γ-Induced Proinflammatory Mediators. Molecules 2022; 27, 3877, 1-17.

Frequently Asked Questions:

What is inflammation scientifically?

Inflammation is an essential defence mechanism for our body's healing process and our body's immune system's response to stimulants from various external factors (ie. UV radiation, pollution, chemicals) and internal factors (ie. lack of sleep, diet, damaged cells). It targets infection and can stimulate tissue repair and regeneration but when it fails to improve quickly it can become chronic which may lead to disease. There is acute and chronic inflammation.

What is a natural anti-inflammatory?

Substances derived from natural sources, such as Omega-3 fatty acids found in fish (eg. salmon, tuna) or natural compounds found in plants and plant extracts, show signs they contribute to the down regulation of inflammation via various pathways including the Nitric oxide (NO), TNF-alpha, IL-1, IL-6 etc. We have recently done pre-clinical university research on a number of plant Cellular Extracts that demonstrated anti-inflammatory and antioxidant activity.

Why managing inflammation is important to skin health?

We believe managing inflammation is an important topic when looking at skin aging. It is thought to speed up aging as well as the skin's ability to protect itself and repair damage. At a molecular level, inflammation triggers a series of interconnected processes with significant implications for skin wellbeing. It is believed that inflammation can make skin age faster by breaking down important components such as collagen, elastin, hyaluronic acid which impacts the skin's ability to maintain its structural integrity. Excessive inflammation during wound healing may impede tissue regeneration, and is believed to accelerate cellular damage and oxidative stress, contributing to the formation of wrinkles and fine lines.